Susceptibility Testing Template Program to create zone templates
Standardised disc diffusion method involves measuring the diameter of zones of inhibition with a ruler, callipers, or with an automated zone reader; however, zones of inhibition may also be measured in relation to zone templates.
The Renaissance artist Giotto de BonDone was able to draw a perfect circle freehand. The less gifted may use this program (known as Giotto2) to generate their zone templates.
Instructions to access the Susceptibility Testing Template Program:
How to add templates to controlled documents
Once the templates have been produced many laboratories need to add them to controlled Standard Operating Procedure (SOP) documents.
This document contains instructions for adding templates to controlled Standard Operating Procedures (SOP): How to add templates to controlled documents.
Quality control of Antimicrobial Susceptibility Testing
The following documents may be useful for tracking Internal Quality Control (IQC) data and also diagnosing IQC problems when performing the EUCAST disc diffusion method
This document contains relevant information to help diagnose problems with quality control data: Quality Control Information
To maintain high quality of susceptibility testing results, IQC should be performed either daily or at least 4 times per week. Keep track of your laboratory’s zone diameter IQC data using the QC disc data tracker. This Excel document allows you to track your QC disc diameter data, included is a help file.
Uncertainty of Measurement
All manual susceptibility testing requires reading of inhibition zones or ellipses. To maintain high quality result reporting, all laboratories should assess the ability of staff to measure zone diameters and MICs. These documents describe the natural and human errors within susceptibility testing methods and provide a template for recording Uncertainty of Measurement (UoM):
For help with any of the susceptibility testing tools on this webpage please contact Mandy Wootton