Additional UK specific susceptibility testing guidance

The EUCAST disk diffusion method has been developed to cover most antimicrobial agent/organism combinations.  A few gaps remain (e.g. Neisseria gonorrhoeae) but these are being actively developed.  The following guidance may help in addressing these gaps:

Susceptibility testing of Neisseria gonorrhoeae

BSAC is currently working with EUCAST to develop a disk diffusion method for Neisseria gonorrhoeae.  Laboratories are advised to continue testing using the BSAC method in the interim. Laboratories are advised to either continue testing using the BSAC disk method & breakpoints in the interim OR perform MIC testing and interpret using EUCAST breakpoints. MIC testing should be performed using Manufacturer’s instructions; in most cases Gradient strips will be employed. Currently, the media recommended for testing Neisseria species by Gradient strip manufacturers is not available in the UK. BSAC are working with manufacturers to resolve this issue.

Anaerobic bacteria
EUCAST is currently developing a method to susceptibility test anaerobic bacteria by disk diffusion. “Laboratories are advised to either continue testing using the BSAC disk method & breakpoints in the interim OR perform MIC testing and interpret using EUCAST breakpoints. MIC testing should be performed using Manufacturer’s instructions; in most cases Gradient strips will be employed. Currently, the media recommended for testing Anaerobic bacteria by Gradient strip manufacturers is not available in the UK. BSAC are working with manufacturers to resolve this issue.”

List of agents to test: This document lists suggested agents to test

Principals of reporting: Guidance for reporting susceptibility testing

COMING SOON …

Validation of any divergence from the EUCAST disk diffusion method:

  • Direct disk diffusion testing in urines and blood cultures
  • MLSB testing in Streptococci at 20mm distance

 

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